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INCREASED CONCENTRATIONS OF GLYCINE IN HYPOXANTHINE‐GUANINE PHOSPHORIBOSYLTRANSFERASE‐DEFICIENT MOUSE NEUROBLASTOMA CELLS 1
Author(s) -
Skaper S. D.,
Seegmiller J. E.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb04437.x-i1
Subject(s) - hypoxanthine guanine phosphoribosyltransferase , phosphoribosyltransferase , glycine , lesch–nyhan syndrome , hypoxanthine , hypoxanthine phosphoribosyltransferase , enzyme , neuroblastoma , biochemistry , intracellular , guanine , purine metabolism , chemistry , microbiology and biotechnology , biology , amino acid , cell culture , genetics , gene , nucleotide , mutant
—The intracellular concentrations of a number of amino acids were compared in a parental line of mouse neuroblastoma cells, a line selected for gross deficiency of the enzyme hypoxanthine‐guanine phosphoribosyltransferase (HGPRT) and in a revertant line containing the enzyme selected from the deficient line. Most prominent was the increased concentration of free glycine and to a lesser extent glutamic acid in enzyme‐deficient cells. The increase in glycine was eliminated after restoration of the HGPRT activity.