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NEUROSECRETORY CELL PROTEIN METABOLISM IN THE LAND SNAIL, OTALA LACTEA
Author(s) -
Loh Y. Peng,
Barker J. L.,
Gainer H.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb04430.x
Subject(s) - snail , anisomycin , biology , protein biosynthesis , polyacrylamide gel electrophoresis , colchicine , land snail , metabolism , biochemistry , gel electrophoresis , cell , microbiology and biotechnology , ecology , genetics , enzyme
—Protein synthesis in an identified molluscan neurosecretory cell of the land snail, Otala lactea was examined using three different types of polyacrylamide gel electrophoresis. Cells taken from active snails synthesized specific low molecular weight proteins while those from aestivated snails did not. Most of the newly synthesized low molecular weight proteins in the active snails were lost from the cell body when the preparations was chased for 19 h in label‐free enriched medium in the presence of anisomycin, an inhibitor of protein synthesis. If colchicine, a blocker of axonal transport, was included in the chase medium, the proteins present following a pulse were largely replaced by smaller molecular weight species. The results suggest that specific low molecular weight proteins are converted to smaller species and then transported from the cell body.