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NEURAMINIDASE‐RELEASABLE SURFACE SIALIC ACID OF CULTURED ASTROBLASTS EXPOSED TO ETHANOL
Author(s) -
Noble E. P.,
Syapin P. J.,
Vigran R.,
Rosenberg A.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb01567.x
Subject(s) - sialic acid , neuraminidase , ethanol , ganglioside , biochemistry , glycoprotein , sialidase , hamster , enzyme , cell culture , chemistry , cell , hydrolysis , neuraminic acid , biology , microbiology and biotechnology , genetics
— Chronic exposure of intact cultured primary hamster astroblasts, clonal line NN, to 100 mM ethanol resulted in significant increases (10–52%) in the releasability of sialic acid from the cell by exogenously added Clostridium perfringens neuraminidase. Enzymatically releasable sialic acid was from the glycoprotein but not the ganglioside fraction of the cell. Addition of ethanol to the medium did not affect the enzymatic hydrolysis of sialyllactose nor did ethanol, over a concentration range of 0–200 mM, alter the enzymatic release of sialic acid from intact NN cells. The total cellular sialic acid content was independent of length of cell exposure to ethanol but varied with the age of the cells. These data suggest that exposure of cells for prolonged periods to ethanol results in steric modification of surface glycoproteins.