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BIOSYNTHESIS OF RAT BRAIN PHOSPHATIDYLCHOLINES FROM INTRACEREBRALLY INJECTED CHOLINE 1
Author(s) -
Arienti G.,
Corazzi L.,
Woelk H.,
Porcellati G.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb01565.x
Subject(s) - choline , phosphorylcholine , phosphatidylcholine , chemistry , in vivo , biochemistry , phospholipid , biology , membrane , microbiology and biotechnology
— [ Me ‐ 3 H] Choline was injected intracerebrally into male rats and the brains immediately removed by particular procedures at regular intervals over the first 1200 s. The incorporation of radioactivity into brain phosphorylcholine, CDP‐choline and phosphatidylcholines was examined and quantitated, in order to investigate the relative roles of net synthesis and base‐exchange reactions for choline incorporation into lipid. The molecular subspecies of phosphatidylcholines were also examined after isotope administration. Phosphorylcholine, CDP‐choline and phosphatidylcholines all became labelled as early as 5 s after the administration of labelled choline. The time course of incorporation of choline into brain lipid is biphasic with two flex points at about 20 and 120 s from the injection. The specific radioactivity of different phosphatidylcholines appears to be different at early and later intervals from injection. The suggestion is made that the base‐exchange pathway for choline incorporation into lipid might be operative in vivo in early periods after administration.