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A BRAIN‐SPECIFIC PROTEIN WITH AFFINITY FOR DNA 1
Author(s) -
Miani N.,
Caniglia A.,
Panetta V.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb01556.x
Subject(s) - isoelectric point , dna , affinity chromatography , biochemistry , glycoprotein , isoelectric focusing , biology , dna binding protein , protein purification , cytosol , specific activity , microbiology and biotechnology , chromatography , chemistry , gene , enzyme , transcription factor
— A procedure has been developed which allows the isolation from rat brain cytosol of a soluble acidic protein, designated DNA‐110 protein, having two basic properties: selective affinity for single‐stranded DNA and immunological specificity to the nervous system. Only two major purification steps, DNA‐cellulose chromatography and affinity chromatography on immunoadsorbents are needed to give apparently pure protein. The purification steps of the DNA‐110 protein have been followed by immunological assay. DNA‐110 has a molecular weight of 68,000 and an isoelectric point of 5.9. It accounts for 1.95% of the total soluble protein and its concentration is 216 μg per g wet weight of rat brain. DNA‐110 is immunologically unrelated to other soluble acidic brain‐specific proteins and glycoproteins.