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FACTORS REQUIRED FOR Ca‐SENSITIVE ACETYLCHOLINE RELEASE FROM CRUDE SYNAPTIC VESICLES
Author(s) -
Hata F.,
Kuo C. H.,
Matsuda T.,
Yoshida H.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb01555.x
Subject(s) - pronase , synaptic vesicle , acetylcholine , chemistry , vesicle , calcium , biophysics , biochemistry , intracellular , trypsin , membrane , egta , biology , endocrinology , enzyme , organic chemistry
— Studies were made on intracellular factors required for the release of ACh from crude synaptic vesicles prepared from rat brain. A factor stimulating ACh release in the presence of calcium ions was found in the cell sap. Cell sap obtained by hypotonic shock of a crude mitochondrial fraction stimulated ACh release from the synaptic vesicles in the presence of ATP and magnesium ions. Calcium ions were essential for its effect and the optimum concentration of calcium was about 10 −4 m . The stimulatory factor in cell sap passed through a cellulose or collodion membrane or ultrafiltration membrane UM 10, but not through that of an ultrafiltration membrane UM 05. The factor was not affected by trypsin or α‐chymotrypsin, but was inactivated by pronase or carboxypeptidase‐A. Thus it seemed to be a peptide‐like substance with a rather low molecular weight. Cyclic‐AMP, cyclic‐GMP, colchicine and vinblastne did not affect ACh release under our experimental conditions.