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THE NUCLEAR DNA CONTENT OF LARGE VENTRAL SPINAL NEURONS
Author(s) -
McIlwain D. L.,
CappsCovey Patsy
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb01551.x
Subject(s) - spinal cord , nucleus , nuclear dna , dna , ploidy , cell nucleus , propidium iodide , biology , anatomy , chemistry , microbiology and biotechnology , biophysics , neuroscience , genetics , apoptosis , programmed cell death , gene , mitochondrial dna
— A nuclear fraction has been obtained from large spinal neurons previously isolated from bovine ventral spinal cord in bulk suspensions. The fraction contained an average of 5.3 ± 0.9 pg DNA/nucleus, indicating a high incidence of diploid nuclei. This conclusion was confirmed by distribution analysis of DNA in propidium iodide‐stained nuclei examined by flow microfluorometry. That technique showed that at least 90% of the nuclei from large spinal neurons are diploid. Mixed, mostly non‐neuronal nuclei derived from many types of cells in the ventral spinal cord contained an average of 5.9 ± 0.6 pg DNA/nucleus, 19% of which possibly possess more than diploid amounts of DNA. The uniform DNA content in nuclei of large spinal neurons and most other types of cells in the ventral spinal cord contrasts sharply with a wide variation (av 26‐fold) in the nuclear volumes of the same cells.