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A RAPID METHOD FOR ASSAYING ENZYMES WHOSE SUBSTRATES AND PRODUCTS DIFFER BY CHARGE. APPLICATION TO BRAIN l ‐GLUTAMATE DECARBOXYLASE
Author(s) -
Chude Obi,
Wu JangYen
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb01547.x
Subject(s) - glutamate decarboxylase , substrate (aquarium) , enzyme , glutamate receptor , chemistry , biochemistry , chromatography , biology , ecology , receptor
— A simple, rapid, sensitive and economical assay technique has been developed for any enzyme whose substrate and product differ by charge. It is based on a combination of rapid vacuum filtration with an ion exchange resin. The technique has been successfully applied to brain glutamate decarboxylase.