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PHOSPHOLIPID COMPOSITION AND [ 14 C]GLYCEROL INCORPORATION INTO GLYCEROLIPIDS OF TOAD RETINA AND BRAIN
Author(s) -
Bazán Haydée E. Pascual,
Bazán N. G.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb00307.x
Subject(s) - phosphatidic acid , phosphatidylinositol , biochemistry , phospholipid , diacylglycerol kinase , biology , biosynthesis , inositol , glycerol , phosphatidylglycerol , diglyceride , chemistry , phosphatidylcholine , fatty acid , membrane , signal transduction , enzyme , receptor , protein kinase c
— The phospholipid composition as well as the in vivo [ 14 C]glycerol uptake in lipids was found to be similar in the toad brain and retina. The choroid lipid labeling was markedly different. An in vitro time‐course study of [ 14 C]glycerol incorporation in toad retina lipids disclosed that under the conditions of these experiments: (1) retina is able to rapidly synthetize phosphatidic acid from the radioactive precursor; (2) the sequence phosphatidic acid‐diacylglycerol‐triacylglycerol operates; (3) a high rate of phosphatidylinositol de novo biosynthesis takes place; (4) phosphoglycerides of choline and of ethanolamine are also heavily labeled after a lag period; (5) in vivo labeling profiles resembled those obtained in vitro mainly regarding phosphatidylinositol biosynthesis; and (6) the presence of glycerol kinase in the CNS is suggested.