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A METHOD FOR THE ISOLATION AND ESTIMATION OF GABA, GLUTAMATE AND GLUTAMINE FROM COMPLEX MIXTURES BY A COMBINATION OF ENZYMIC INTERCONVERSION AND FORMATION OF 1‐DIMETHYLAMINONAPHTHALENE‐5‐SULPHONYLBUTYROLACTAM
Author(s) -
Strang R. H. C.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb07689.x
Subject(s) - glutamine , glutamate receptor , chemistry , isolation (microbiology) , amino acid , polarity (international relations) , chromatography , biochemistry , biology , bioinformatics , receptor , cell
— A method is presented which allows the isolation and estimation of GABA, glutamate and glutamine in extracts of nervous tissue. It depends on the enzymic conversion of the last two compounds to GABA, before isolating the GABA as DNS‐γ‐butyrolactam. The low polarity of this compound makes this possible without the use of chromatography. Furthermore, the specific radioactivities of these 3 compounds in a complex mixture may be found without elaborate separation procedures. Details are given of optimum reaction conditions, and comparisons made with other methods of estimation of these amino acids.