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FACTORS INFLUENCING THE EFFLUX OF [ 3 H]GAMMAAMINOBUTYRIC ACID FROM SATELLITE GLIAL CELLS IN RAT SENSORY GANGLIA
Author(s) -
Minchin M. C. W.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb07676.x
Subject(s) - efflux , veratridine , chemistry , biophysics , biochemistry , batrachotoxin , choline , biology , sodium , sodium channel , organic chemistry
— The spontaneous efflux of [ 3 H]GABA from the satellite glial cells of rat dorsal root ganglia and the efflux evoked by 64 mM‐K + were studied in the presence of 10 ‐5 M‐amino‐oxyacetic acid and found not to be affected by 10 ‐4 M‐D 600 or by elevated (9.6mM) Ca 2+ in the absence of Mg 2+ . [ 3 H]GABA efflux was increased by replacing sodium ions in the washing medium by choline ions and 64 mM‐K + failed to increase the efflux further. The drugs veratridine (10 ‐6 and 10 ‐4 M) and batrachotoxin (10 ‐8 and 10 ‐6 M) failed to alter the spontaneous efflux of [ 3 H]GABA from the glial cells. A variety of compounds, including amino acids, a GABA analogue and a GABA antagonist were tested for their ability to affect [ 3 H]GABA efflux. The results indicated that compounds which inhibit GABA uptake into glial cells were also able to stimulate [ 3 H]GABA efflux from these cells. The results are discussed with reference to possible mechanisms involved in the release of GABA from glial cells.