Premium
A RELATIONSHIP OF N ‐ACETYLASPARTATE BIOSYNTHESIS TO NEURONAL PROTEIN SYNTHESIS 1 2
Author(s) -
Clarke D. D.,
Greenfield S.,
Dicker E.,
Tirri L. J.,
Ronan E. J.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb07665.x
Subject(s) - moiety , sodium acetate , ethanol , chemistry , specific activity , biosynthesis , biochemistry , sodium , microsome , rna , protein biosynthesis , stereochemistry , chromatography , enzyme , organic chemistry , gene
— A detailed time study of the incorporation of label from sodium‐[1‐ 14 C]acetate, [1‐ 14 C]ethanol, and [2‐ 14 C]glucose into the aspartyl moiety of N ‐acetylaspartic acid (NAA) was conducted. As expected the specific activity of aspartate increased rapidly with time and peaked within 15‐20 min after which it fell sharply; but significantly, that of the aspartyl moiety of NAA rose very slowly even after the specific activity of aspartate had fallen to less than 1 per cent of the peak values. A rat brain microsomal free supernatant preparation was shown enzymatically to incorporate label from sodium‐[1‐ 14 C]acetate into the t‐RNA fraction from which was isolated N ‐[1‐ 14 C]acetylaspartic acid. From these observations we were inclined to speculate that NAA‐t‐RNA may serve as an initiator of neuronal protein synthesis.