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FUNCTIONAL COMPARTMENTS OF ADENINE NUCLEOTIDES SERVING AS PRECURSORS OF ADENOSINE 3′,5′‐MONOPHOSPHATE IN MOUSE CEREBRAL CORTEX
Author(s) -
Skolnick P.,
Daly J. W.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb07661.x
Subject(s) - veratridine , adenosine , inosine , chemistry , adenosine monophosphate , stimulation , adenine nucleotide , norepinephrine , biochemistry , medicine , endocrinology , nucleotide , biology , sodium , dopamine , organic chemistry , sodium channel , gene
Abstract— Cyclic AMP accumulates in cerebral cortical slices from the C57B1/6J mouse incubated with the following stimulatory agents: norepinephrine, adenosine, veratridine and adenosine‐biogenic amine combinations. The results with slices labelled with radioactive adenine or adenosine provide evidence for the existence of distinct functional compartments of adenine nuclcotides which serve as precursors of cyclic AMP on stimulation with specific agents. Thus, in slices labelled with [ 14 C]adenine or [ 3 H]adenosine the ratio of [ 14 C] to [ 3 H]cyclic AMP was dependent on the stimulatory agent; with veratridinc the ratio was 1.4 while with adenosine the ratio was 3.0. In addition, a greater than 2‐fold difference in the ratio of endogenous/radioactive cyclic AMP was observed in adenine or adenosine‐labelled slices after incubation with veratridine, norepinephrine, adenosine or adenosine‐amine combinations; the lowest ratios after stimulation with veratridine and the highest after adenosine or adenosine‐amine combinations. The high ratio observed with adenosine was in part due to a quite marked incorporation of the stimulant, adenosine, into the accumulating cyclic AMP. Such distinct functional compartments of cyclic AMP precursors may represent different cell types and/or morphological entities within one cell type.