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RATE OF STEROL FORMATION BY RAT BRAIN GLIA AND NEURONS IN VITRO AND IN VIVO
Author(s) -
Jones J. P.,
Nicholas H. J.,
Ramsey R. B.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb07637.x
Subject(s) - mevalonic acid , in vivo , sterol , biochemistry , squalene , terpenoid , in vitro , biology , metabolism , demethylation , neuroglia , geranylgeraniol , chemistry , incubation , biosynthesis , cholesterol , enzyme , endocrinology , central nervous system , gene expression , microbiology and biotechnology , gene , dna methylation
— The ability of 11‐day‐old rat glial and neuronal cells to biosynthesize sterol was studied as a function of time in vivo and in vitro . The in vitro experiments utilized [2‐ 14 C]mevalonic acid as precursor. Glial‐enriched cell preparations demonstrated a greater ability to incorporate [2‐ 14 C]mevalonic acid into isoprenoid material than did neuronal‐enriched preparations. Approximately 4 h were required for maximal uptake of labelled mevalonate by the glial preparations. Further metabolism of the isoprenoid material, involving squalene turnover and sterol demethylation, was still evident even after 15 h of incubation. In vivo , sterol biosynthesis was studied by intraperitoneal injection of sodium [2‐ 14 C]acetate and [U‐ 14 C]glucose, sacrifice of the animals at 2 or 24 h, subsequent isolation of glial‐ and neuronal‐cell enriched fractions and analysis of labelled isoprenoid material. Glial‐enriched fractions again contained the bulk of the labelled isoprenoid material.