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SUBCELLULAR DISTRIBUTION OF SUBSTANCE P IN BOVINE HYPOTHALAMUS AND SUBSTANTIA NIGRA
Author(s) -
Duffy M. J.,
Mulhall D.,
Powell D.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb06971.x
Subject(s) - substantia nigra , radioimmunoassay , differential centrifugation , hypothalamus , chemistry , centrifugation , mitochondrion , cell fractionation , substance p , enzyme , biology , microbiology and biotechnology , biochemistry , endocrinology , dopamine , neuropeptide , receptor , dopaminergic
— Subcellular distribution of substance P as measured by radioimmunoassay was studied in fractions and subfractions from bovine hypothalamus and substantia nigra. Most of the substance P was found in the crude mitochondrial fraction. Subfractionation of the crude mitochondrial fraction by density gradient centrifugation showed most of substance P was present in nerve ending particles. Subcellular particle integrity was assessed using enzyme markers.