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THE BINDING OF 5‐HYDROXYTRYPTAMINE TO BUTANOL EXTRACTS OF RAT BRAIN STEM
Author(s) -
Godwin S. F. R.,
Sneddon J. M.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb06967.x
Subject(s) - butanol , sephadex , chemistry , chloroform , chromatography , methanol , elution , selectivity , receptor , biochemistry , ethanol , enzyme , organic chemistry , catalysis
— When butanol‐water extracts of rat brain stem were incubated with [ 3 H]5‐HT, (5 × 10 −7 m ), and the components resolved by chromatography on LH 20 Sephadex, a peak representing approximately 70% of the eluted radioactivity was found in chloroform‐methanol 4:1. The peak was not found in identically prepared extracts from rat diaphragm, neither was a similar peak found when brain extracts were incubated with [ 14 C]ACh (10 −6 m ), suggesting a degree of selectivity. Binding was not saturated at concentrations of 5 × 10 −5 m ‐5‐HT. The binding was highly sensitive to the presence of water, requiring about 15% (v/v) for optimum binding. The implications of these findings are discussed in terms of a possible ‘5‐HT receptor’.