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DISTRIBUTION OF BIOGENIC AMINES AND RELATED ENZYMES IN THE RAT PITUITARY GLAND
Author(s) -
Saavedra J. M.,
Palkovits M.,
Kizer J. S.,
Brownstein M.,
Zivin J. A.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb06962.x
Subject(s) - endocrinology , medicine , dopamine , pituitary gland , monoamine oxidase , tryptophan hydroxylase , phenylethanolamine , serotonin , catecholamine , lobe , tryptamine , tyrosine hydroxylase , chemistry , anterior pituitary , biology , enzyme , anatomy , biochemistry , hormone , serotonergic , receptor
— Biogenic amines and related enzymes were quantitatively measured in the pituitary gland of the rat. The sensitivity of the assays used allows the determinations to be performed in single pituitary lobes. Relatively high values of histamine and serotonin were found in all three lobes, with higher amounts in the posterior and intermediate lobes. Highest catecholamine concentrations were detected in the posterior lobe, and only very low amounts of dopamine were measured in the anterior lobe. Throughout the gland, norepinephrine concentrations were low, about one‐tenth that of dopamine. Tryptamine could not be detected. High levels of A and B monoamine oxidase were found in all three pituitary lobes. Tyrosine hydroxylase activity was measured in the posterior and intermediate lobes, but was not detected in the anterior lobe. Tryptophan hydroxylase was present in all three pituitary lobes. A relatively low catechol‐ O ‐methyltransferase activity was found in the anterior lobe, and none was detected in the intermediate and posterior lobe. Choline acetyltransferase, dopamine‐β‐hydroxylase and phenylethanolamine‐ N ‐methyltransferase activities could not be detected.