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METABOLISM OF POLYAMINES IN MOUSE NEUROBLASTOMA CELLS IN CULTURE: 1 FORMATION OF GABA AND PUTREANINE
Author(s) -
Kremzner L. T.,
Hiller J. M.,
Simon E. J.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb04423.x
Subject(s) - spermidine , putrescine , spermine , diamine oxidase , polyamine oxidase , biochemistry , metabolism , ornithine , polyamine , biology , chemistry , microbiology and biotechnology , enzyme , amino acid , arginine
—Polyamine metabolism of mouse neuroblastoma cells grown in culture was studied with special reference to the synthesis of GABA from putrescine and putreanine from spermidine. This study shows that neuroblastoma cells in the presence of a complete culture medium containing calf serum readily metabolized [ 14 C]putrescine to GABA; the rate of synthesis is similar to the rate of synthesis of spermidine from putrescine. In the absence of serum the conversion of putrescine to GABA is minimal. In the presence of serum GABA formation is completely inhibited by the diamine oxidase inhibitor aminoguanidine. GABA synthesis does not occur in the absence of cells. The GABA synthesized is not readily metabolized to succinate or homocarnosine. Mouse neuroblastoma cells metabolized [ 14 C]ornithine to putrescine, GABA, and spermidine. Spermidine was metabolized to putrescine, putreanine and spermine.