Premium
THE REACTION OF CHOLINE AND 3,3‐DIMETHYL‐1‐BUTANOL WITH THE ACETYLENZYME FROM ACETYLCHOLINESTERASE
Author(s) -
Dawson R. M.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb04408.x
Subject(s) - acetylcholinesterase , chemistry , choline , tetramethylammonium , acetylthiocholine , acetic acid , choline chloride , hydrolysis , acetylcholine , substrate (aquarium) , ethanol , cholinesterase , medicinal chemistry , nuclear chemistry , inorganic chemistry , organic chemistry , biochemistry , enzyme , ion , aché , biology , ecology , pharmacology , endocrinology
—A comparison was made of the manner in which choline chloride and 3,3‐dimethyl‐1‐butanol react with the acetylenzyme formed during the hydrolysis of esters of acetic acid by acetylcholinesterase. Acetylcholine and acetylthiocholine were the substrates. The ratio of the formation of alkyl acetate to that of acetic acid increased linearly with the concentration of dimethylbutanol, but approached a limiting value as the concentration of choline was increased. Total enzymic activity was inhibited by choline and activated slightly by dimethylbutanol. The effects of varying ionic strength, pH and substrate concentration were examined. The effects of tetraethyl‐ and tetramethylammonium ions on the reaction of dimethylbutanol with the acetylenzyme were also studied. The results suggest that dimethylbutanol and choline bind to different regions of the active site. A mechanism for the reaction of choline and substrate with acetylcholinesterase is suggested.