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BIOCHEMICAL CHANGES IN RAT BRAIN ASSOCIATED WITH THE DEVELOPMENT OF THE BLOOD‐BRAIN BARRIER
Author(s) -
Sessa G.,
Perez Maria M.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb04407.x
Subject(s) - monoamine oxidase , endocrinology , medicine , enzyme , alkaline phosphatase , blood–brain barrier , 5' nucleotidase , specific activity , brain function , biology , central nervous system , nucleotidase , enzyme assay , chemistry , biochemistry , neuroscience
—Subcellular fractions from brains of 5, 10, 13, 16, 21, 30 day‐old and adult rats were prepared. Protein content and various enzyme activities were assayed in all fractions and brain homogenates. γ‐Glutamyl transpeptidase activity and 5′‐nucleotidase were very low at 5 days of life but steadily increased, reaching adult concentrations at about 30 days after birth. Alkaline phosphatase, instead slowly decreased with maturation, while monoamine oxidase after an initial decrease, increased rapidly to adult levels. The relation between the appearance of enzymatic activity in brain and the blood‐brain barrier function is discussed.