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THE DISPOSITION OF [24‐ 3 H]CEREBROSTEROL IN DEVELOPING RAT BRAIN *
Author(s) -
Lin Y. Y.,
Smith L. L.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb04385.x
Subject(s) - microsome , myelin , free nerve ending , catabolism , sterol , metabolism , cholesterol , chemistry , in vivo , mitochondrion , microsoma , biochemistry , endocrinology , medicine , biology , in vitro , central nervous system , microbiology and biotechnology
—The uptake into subcellular fractions of developing rat brain in vivo of intracerebrally injected [4‐ 14 C]cholesterol, [24‐ 3 H]cerebrosterol, and [24‐ 3 H]24‐epicerebrosterol was measured for periods up to 30 days following administration. [4‐ 14 C]cholesterol was accumulated rapidly in nuclei, nerve endings, and microsomes, more slowly in myelin and mitochondria. [24‐ 3 H]cerebrosterol was accumulated rapidly in myelin, nerve endings, and microsomes, more slowly in nuclei and mitochondria. The uptake of [24‐ 3 H]24‐epicerebrosterol was essentially the same as that of [24‐ 3 H]cerebrosterol. Ratios of radioactivities of [24‐ 3 H]cerebrosterol and [4‐ 14 C]cholesterol accentuated the early accumulation of [24‐ 3 H]cerebrosterol in myelin, nerve endings, and microsomes, and declining 3 H: 14 C ratios disclosed the rapid elimination of [24‐ 3 H]cerebrosterol and [24‐ 3 H]24‐epicerebrosterol relative to [4‐ 14 C]cholesterol in nerve endings and microsomes. The data suggest that the removal of [24‐ 3 H]cerebrosterol from brain results from an enzymic metabolism of the sterol, therefore that cerebrosterol exists in brain in a dynamic state of biosynthesis and catabolism.