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FRACTIONATION OF SPECIFIC MITOCHONDRIAL AND CYTOPLASMIC tRNAs OBTAINED FROM CALF BRAIN 1
Author(s) -
Borkowski T.,
BrzuszkiewiczŻarnowska H.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb04381.x
Subject(s) - cytoplasm , transfer rna , incubation , biochemistry , mitochondrion , biology , cell fractionation , fractionation , chromatography , rna , chemistry , enzyme , gene
—Total tRNA fractions were isolated from pure mitochondrial and cytoplasmic calf brain preparations. After incubation with homologous crude preparations of aminoacyl‐tRNA ligases in the presence of [ 14 C]‐glutamic acid, tRNAs were separated chromatographically on BD‐cellulose columns and in reversed phase chromatography systems. In both of the methods used, cytoplasmic tRNA preparations revealed a larger number of radioactivity peaks. In experiments with double labelling, five radioactivity peaks for cytoplasmic glutamyl‐tRNAs corresponded to only three mitochondrial glutamyl‐tRNA fractions. The results imply the presence of isoaccepting species of tRNA in brain.