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EFFECTS OF AMINO‐OXYACETIC ACID, ETHANOLAMINE‐O‐SULPHATE AND GABA ON THE CONTENTS OF GABA AND VARIOUS AMINES IN BRAIN SLICES
Author(s) -
Starr M. S.,
Tanner T.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb04370.x
Subject(s) - dopamine , chemistry , ethanolamine , medicine , endocrinology , serotonin , hypothalamus , midbrain , amino acid , biogenic amine , norepinephrine , neurotransmitter , biochemistry , biology , central nervous system , receptor
—The effects of amino‐oxyacetic acid, ethanolamine‐O‐sulphate and γ‐aminobutyric acid (GABA) on the contents of GABA, noradrenaline, dopamine and serotonin (5‐HT) in slices of rat hypothalamus and midbrain were studied in vitro using a simultaneous fluorimetric assay procedure. Following control incubations the levels of 5‐HT were raised, while the levels of the other substances remained steady. Amino‐oxyacetic acid caused a reduction in the contents of noradrenaline and 5‐HT, but had no effect on either GABA or dopamine. Ethanolamine‐O‐sulphate both raised the GABA content and lowered the noradrenaline content of slices, while the levels of dopamine and 5‐HT were not altered. The presence of GABA in the incubation medium produced complex changes in these levels, depending both on the dose of GABA used and the brain area studied. In the hypothalamus, 0·07 m m ‐GABA caused an elevation in 5‐HT, a drop in noradrenaline, and no change in either GABA or dopamine. With 5 m m ‐GABA, the noradrenaline level was raised slightly above control values and the endogenous GABA level doubled, while 5‐HT and dopamine levels were not different from controls. Similar changes in 5‐HT and GABA contents were observed with midbrain slices, but noradrenaline and dopamine were not affected. The possible modes of action of amino‐oxyacetic acid and ethanolamine‐O‐sulphate on the amino acid and amine systems in the brain are discussed.