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FRACTIONATION OF RNA FROM BRAIN SYNAPTOSOMES AND CYTOPLASMIC SUBCELLULAR FRACTIONS
Author(s) -
Cupello A.,
Hydén H.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb04337.x
Subject(s) - synaptosome , rna , electrophoresis , cytoplasm , mitochondrion , fractionation , microsome , cell fractionation , biology , biochemistry , chemistry , microbiology and biotechnology , chromatography , in vitro , enzyme , gene
—RNA from rat brain synaptosomes, mitochondria and microsomes was analysed by gel electrophoresis under conditions allowing good resolution in three different molecular weight ranges: 4s‐16s, 16s‐28s and >28s. Two synaptosome specific RNA bands were found, one with comparatively low molecular weight (8‐9 × 10 4 Daltons) and another very large (s E > 60s). RNA species with electrophoretic characteristics similar to those reported for liver mitochondrial RNA were found in brain mitochondria. From the electrophoretic data their mean geometric radii were determined.