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THE SUBCELLULAR LOCALIZATION OF CARBAMYLCHOLINE‐STIMULATED PHOSPHATIDYL INOSITOL TURNOVER IN RAT CEREBRAL CORTEX IN VIVO
Author(s) -
Lunt G. G.,
Pickard M. R.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb03899.x
Subject(s) - inositol , cerebral cortex , in vivo , chemistry , endocrinology , medicine , cortex (anatomy) , biochemistry , biology , neuroscience , receptor , microbiology and biotechnology
— The intraventricular injection of 40 μCi of 32 P i (carrier free) into adult rats resulted in maximum incorporation of 32 P i into the phosphatidyl inositol of the whole cortex after 20 h. A further intraventricular injection of 2 nmol carbamylcholine plus 0.02 nmol eserine resulted in a 23% decrease in the specific activity of phosphatidyl inositol after 20 min. The specific radioactivities of phosphatidyl choline, phosphatidyl ethanolarmine and phosphatidyl serine were not changed. Cerebral cortex from rats treated in this way was subjected to an extensive subcellular fractionation. It was found that the specific radioactivity of the phosphatidyl inositol of the synaptic vesicle fraction showed a reduction of 60%. No other fractions showed effects of this magnitude.