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RESPIRATION IN IMMATURE RAT BRAIN MITOCHONDRIA
Author(s) -
Holtzman D.,
Moore C. L.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb03670.x
Subject(s) - respiration , mitochondrion , nad+ kinase , respiratory system , substrate (aquarium) , respiratory rate , biology , glutamate receptor , endocrinology , respiration rate , medicine , biochemistry , chemistry , anatomy , enzyme , heart rate , receptor , ecology , blood pressure
—Respiration was studied polarographically in mitochondria isolated from immature rat cerebral hemispheres. Respiratory rates are compared as a function of age, substrate, and the requirement for a phosphate acceptor. 1. All respiratory rates are low in the first week of life. These rates increase during the first month and then decline to about the newborn rate by 5 weeks of age. 2. With the NAD‐linked substrate pair, glutamate and malate, the changes with age are significant only for the rate of ADP‐dependent respiration. With succinate as substrate, significant age‐dependent changes in respiration occur only in ADP‐independent respiration. 3. In mitochondria from animals less than five weeks of age, the ADP‐dependent respiratory rate is significantly greater with the NAD‐linked substrate pair than with succinate. In mitochondria from older animals, both ADP‐dependent and ADP‐independent rates are greater with succinate.