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COMPARTMENTATION OF AMINO ACID METABOLISM IN THE RAT POSTERIOR PITUITARY
Author(s) -
Minchin M. C. W.,
Beart P. M.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb03651.x
Subject(s) - glutamine , glutamate receptor , metabolism , amino acid , biochemistry , pituitary gland , posterior pituitary , carbohydrate metabolism , chemistry , medicine , glutamic acid , biology , endocrinology , hormone , receptor
—Isolated rat posterior pituitary glands were incubated with [ 14 C]glucose or [ 14 C]acetate and the incorporation of radioactivity into several amino acids was followed. The results indicated that radioactivity was incorporated from [ 14 C]glucose into a large pool of glutamate which appeared to be responsible for a large proportion of GABA synthesis in the gland. The specific activity of glutamine was always less than that of glutamate when [ 14 C]glucose was the precursor employed, whereas [ 14 C]acetate labelled a glutamate pool which had approximately the same specific activity as that of glutamine. The results are discussed with reference to the compartmentation of amino acid metabolism in the nervous system.