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HIGH AFFINITY UPTAKE OF l ‐GLUTAMINE IN RAT BRAIN SLICES
Author(s) -
Balcar V. J.,
Johnston G. A. R.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb03650.x
Subject(s) - glutamine , asparagine , ouabain , glutamate receptor , chemistry , biochemistry , sodium , amino acid , glutamine synthetase , biophysics , biology , receptor , organic chemistry
— l ‐Glutamine is taken up into rat brain slices by a specific‘high affinity’uptake system ( K m 52 μ m ) which is not influenced by high concentrations of l ‐glutamate and l ‐asparagine. The uptake system appears to be associated with cellular structures that do not survive homogenization under conditions which yield synaptosomes. The‘high affinity’uptake of glutamine is dependent on the external sodium ion concentration and can be inhibited by p ‐chloromercuriphenylsulphonate, amino‐oxyacetic acid, ouabain, dibenamine and allylglycine. The effects of several inhibitors indicate that l ‐asparagine uptake is mediated by a system different from the‘high affinity’system mediating l ‐glutamine uptake.