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NEURONAL AND GLIAL SYSTEMS FOR γ‐AMINOBUTYRIC ACID TRANSPORT
Author(s) -
Sellstroum Å.,
Hamberger A.
Publication year - 1975
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1975.tb03646.x
Subject(s) - picrotoxin , aminobutyric acid , bicuculline , strychnine , chemistry , calcium , potassium , sodium , biochemistry , biophysics , gamma aminobutyric acid , biology , gabaa receptor , receptor , organic chemistry
—The uptake of [2,3‐ 3 H]γ‐aminobutyric acid (GABA) by bulk prepared neuronal perikarya, nerve endings and glial cells has been studied in an in vitro ‐system. The uptake in the different fractions had a similar dependence for sodium, potassium and magnesium. Calcium stimulated the synaptosomal GABA uptake at concentrations which inhibited the glial uptake. Bicuculline strongly inhibited the uptake in all fractions. Picrotoxin and strychnine had little effect on the neuronal uptake whereas glial uptake was stimulated. l ‐2,4‐di‐aminobutyric acid and chlorpromazine inhibited GABA uptake in all fractions. The effect of cyclic AMP was not significant.