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CHANGES IN THE NEURONAL PROTEIN LABELLING INDUCED BY POTASSIUM IONS IN VIVO(SPREADING DEPRESSION)
Author(s) -
KŘIVÁNEK J.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb12225.x
Subject(s) - in vivo , cortical spreading depression , potassium , amino acid , chemistry , cerebral cortex , extracellular , cortex (anatomy) , biophysics , biochemistry , incubation , labelling , leucine , biology , medicine , endocrinology , neuroscience , microbiology and biotechnology , organic chemistry , migraine
— Inhibition of incorporation of labelled leucine into the protein of the cerebral cortex induced by potassium ions in vivo (spreading depression) was analysed in isolated neuronal perikarya and compared with that in the whole cortex. Partition of proteins and amino acids and their radioactivities between tissue (cells) and incubation and disintegration media were checked at various steps of the separation procedure. The effect of potassium ions elicited in vivo persisted through all the steps of separation through to the neuronal perikarya pellets. The inhibition of amino acid incorporation was found to be less pronounced in perikarya than in the whole cortex. The validity of the separation method for the study of metabolic changes induced in vivo and possible participation of the glial cells in cerebral cortical responses to enhanced extracellular potassium are discussed.