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PHOSPHOINOSITIDE METABOLISM IN RAT SUPERIOR CERVICAL GANGLION, VAGUS and PHRENIC NERVE: EFFECTS OF ELECTRICAL STIMULATION and VARIOUS BLOCKING AGENTS 1
Author(s) -
White G. L.,
Schellhase H. U.,
Hawthorne J. N.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb12191.x
Subject(s) - superior cervical ganglion , vagus nerve , stimulation , ouabain , labelling , phrenic nerve , chemistry , tetrodotoxin , superior laryngeal nerve , endocrinology , medicine , biochemistry , biology , sodium , respiratory system , organic chemistry
— Paired vagus nerves, phrenic nerves or superior cervical ganglia from rats were incubated at 37 C for various times in a simple salt solution containing glucose and 32 P i . One of the pair was usually stimulated electrically for 30 or 60 min. Stimulation of vagus nerve for 30 min increased phosphate incorporation into all the phospholipids studied but the increase was significant only in the case of triphos‐phoinositide and diphosphoinositide. This increase was not accompanied by increased labelling of the nucleotide labile phosphate pool. Tetrodotoxin at concentrations sufficient to block transmission had no effect upon phospholipid labelling in vagus or phrenic nerve. Ouabain at blocking concentration did not affect polyphosphoinositide metabolism in vagus nerve but increased [ 32 P]labelling of the other phospholipids. Hemicholinium‐3 increased the labelling of all three phosphoinositides in the sympathetic ganglia but the increase in phosphatidylinositol labelling due to electrical stimulation was not seen in the presence of this inhibitor.