Premium
CEREBRAL ANOXIA: EFFECT ON TRANSCRIPTION AND TRANSLATION 1
Author(s) -
Yanagihara T.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb12186.x
Subject(s) - anoxic waters , protein biosynthesis , transcription (linguistics) , rna polymerase , rna , biology , in vitro , biochemistry , translation (biology) , period (music) , microbiology and biotechnology , chemistry , messenger rna , gene , physics , acoustics , ecology , philosophy , linguistics
— The activity of DNA‐dependent RNA polymerase and the synthesis of microsomal protein were investigated after various periods of anoxic condition produced with rabbit brain in an in vitro experimental model. There was prompt inhibition of protein synthesis even after an anoxic period of 5 min, and inhibition was more than 80 per cent after an anoxic period of 30 min. However, RNA polymerase activity was retained during the early stage of anoxia, but definite inhibition appeared after an anoxic period of 15 min. Comparisons with other available information suggest that the inhibition of protein synthesis observed with brain slices is closely related to their polysomal function, that irreversibility of inhibition of protein synthesis might be related to the involvement of nuclear RNA synthesizing mechanism, and that these can occur both in the neuronal and glial elements.