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FACTORS AFFECTING THE SPONTANEOUS RELEASE OF [ 3 H] γ‐AMINOBUTYRIC ACID FROM THE FROG RETINA IN VITRO
Author(s) -
Kennedy A. J.,
Voaden Mary J.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb12179.x
Subject(s) - ouabain , stimulation , potassium , efflux , calcium , aminobutyric acid , retina , chemistry , darkness , aminooxyacetic acid , biophysics , endocrinology , medicine , gamma aminobutyric acid , biochemistry , sodium , biology , neuroscience , enzyme , receptor , botany , organic chemistry
— The spontaneous efflux of [ 3 H]GABA and its metabolites from the frog retina has been studied. The efflux of radioactivity was multiphasic in the presence or absence of amino‐oxyacetic acid (AOAA), an inhibitor of GABA metabolism, and was not affected by light or dark adaptation. Strong retention of radioactivity was evident in the presence of AOAA, about 90% of the label remaining in the tissue after 4 h superfusion. Under these conditions, increases in the rate of release of radioactivity were evoked by electrical stimulation, 40 m m ‐potassium. unlabelled GABA (5 m m ), ouabain (5 × 10 −5 m ) and the absence of calcium. The amount of [ 3 H]GABA released by electrical stimulation was not markedly calcium dependent, whereas the response to 40 m m ‐potassium was reduced by 96% in the absence of calcium.