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PERMEABILITY OF MITOCHONDRIA FROM RAT BRAIN AND RAT LIVER TO GABA
Author(s) -
Brand M. D.,
Chappell J. B.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb12177.x
Subject(s) - mitochondrion , glutamate receptor , nad+ kinase , isolated brain , stimulation , biochemistry , chemistry , biology , mitochondrial matrix , endocrinology , cytosol , enzyme , receptor
— For the GABA shunt to operate in rivo , GABA must be able to enter brain mitochondria. GABA causes reduction of intra‐mitochondrial NAD + ; glutamate or 2‐oxoglutarate stimulate this reduction at concentrations at which they do not themselves cause reduction. This stimulation is not abolished by Triton X‐100. The rates of swelling of brain and liver mitochondria are similar in iso‐osmotic GABA and in several analogues. The rate of swelling is proportional to the concentration of GABA in the iso‐osmotic suspension medium. GABA penetrates 60% of the mitochondrial matrix volume, this value is unaffected by energizing the mitochondria. The activity of GABA‐oxoglutarate aminotransferase is not latent. We conclude that GABA diffuses into both brain and liver mitochondria as a species with no net charge at rates which are able to sustain maximum activity of the GABA shunt.