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MULTIPLE DISC GEL BANDS OF MITOCHONDRIAL CREATINE AND ADENYLATE KINASES 1
Author(s) -
Lapin Evelyn,
Maker H. S.,
Lehrer G. M.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb12172.x
Subject(s) - adenylate kinase , creatine kinase , mitochondrion , kinase , biochemistry , polyacrylamide gel electrophoresis , electrophoresis , microbiology and biotechnology , fraction (chemistry) , creatine , biology , chemistry , enzyme , chromatography
— The activities and electrophoretic patterns of creatine and adenylate kinases in the mitochondrial and high speed supernatant fractions of adult mouse brain were determined. Approximately 22 per cent of the activities of both kinases is firmly bound to the mitochondria. On acrylamide gel electrophoresis of creatine kinase, in addition to the major band previously described, there were several other bands found. Although present in both the mitochondrial and supernatant fractions these additional protein bands with creatine kinase activity were significantly more intense in the mitochondrial fraction. There was only onesecondary band of adenylate kinase activity in the mitochondrial fraction but additional bands were found in the soluble fraction.