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THE FATTY ACID COMPOSITION OF SPHINGOLIPIDS FROM BOVINE CNS AXONS AND MYELIN 1
Author(s) -
DeVries G. H.,
Norton W. T.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb11587.x
Subject(s) - sphingomyelin , myelin , sphingolipid , galactolipids , cerebroside , biochemistry , chemistry , fatty acid , galactolipid , axon , biology , cholesterol , phospholipid , central nervous system , anatomy , endocrinology , membrane , chloroplast , gene
— Cerebrosides, sulphatides and sphingomyelin were isolated from bovine CNS myelin and from myelin‐free axons derived from myelinated axons. The fatty acid composition of each sphingolipid was determined by gas‐liquid chromatography of the fatty acid methyl esters. In each case the fatty acids of the axonal sphingolipids were of shorter average chain length than those from the corresponding myelin lipids. These differences, however, were small and the fatty acids of the axonal cerebrosides and sulphatides were similar in average chain length to those reported previously for bovine myelin. The principal unsubstituted acid of both cerebroside and sulphatide from axons was 24: 1, with the total long chain acids (> C 18 ) amounting to 80 and 85 per cent, respectively. The corresponding figures for myelin galactolipids were 94 and 95 per cent long chain acids. The principal α‐hydroxy acid of both axonal galactolipids was 24 h:0, with cerebroside having 80 per cent and sulphatide 92 per cent long chain acids, compared to the figures of 87 and 97 per cent for the corresponding myelin lipids. In axonal sphingomyelin the major acid was 18:0 (compared to 24:1 in myelin) and the long chain acids were 61 per cent of the total vs 76 per cent of the total for myelin sphingomyelin. The non‐identity of axonal and myelin sphingolipid fatty acids substantiates the belief that they are intrinsic axonal constituents. These findings do not rule out the possibility of a close metabolic relationship between the sphingolipids of the axon and its myelin sheath.