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MEASUREMENT OF THE RATE OF GLUCOSE UTILIZATION BY RAT BRAIN IN VIVO
Author(s) -
Hawkins R. A.,
Miller A. L.,
Cremer Jill E.,
Veech R. L.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb10743.x
Subject(s) - pentobarbital , metabolite , chemistry , in vivo , femoral artery , plasma glucose , sodium , medicine , anesthesia , endocrinology , ether anesthesia , chromatography , biochemistry , biology , insulin , microbiology and biotechnology , organic chemistry
— A method is described by which the rate of glucose utilization by whole brain of conscious rats may be measured. The basis is the uptake of 14 C derived front [2‐ 14 C] glucose into the acid‐soluble metabolite pool of brain. Catheters are placed in the femoral artery and vein under light ether anesthesia. After full recovery of consciousness a single intravenous injection of [2‐ 14 C] glucose is given and arterial blood samples taken at intervals. Simultaneous with the last sample the brain is removed and frozen within 1 s. The accumulation of 14 C into the acid‐soluble metabilite pool is measured and the rate of glucose utilization is calculated according to the equation:The integral is calculated from the plasma glucose specific activity curve and evidence is presented to justify this procedure. The rate of glucose utilization measured by this method was 0·62 μmol/min per g in conscious rats and 0·28 μmol/min per g in sodium pentobarbital anesthetized rats.