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IMMUNOLOGICAL APPROACH TO THE CHARACTERIZATION OF CHOLINERGIC VESICULAR PROTEIN
Author(s) -
Ulmar G.,
Whittaker V. P.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb07611.x
Subject(s) - ouchterlony double immunodiffusion , torpedo , vesicle , antiserum , cholinergic , immunoprecipitation , biochemistry , biology , precipitin , immunodiffusion , vitellogenin , synaptic vesicle , immunochemistry , chemistry , microbiology and biotechnology , antibody , immunology , membrane , endocrinology , acetylcholine receptor , receptor , gene
— Rabbit antisera have been prepared against whole cholinergic vesicles purified from the electric organ of Torpedo marmorata. The sera contain two major and two minor precipitating systems against membranous proteins, as revealed by Ouchterlony diffusion. No immunoprecipitation could be detected against the soluble vesicle protein constituent ‘vesiculin’. Fractions from cephalopod, amphibian and mammalian neural tissue were shown to exhibit no immunochemical homology with Torpedo cholinergic vesicle proteins.