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TURNOVER OF PROTEIN PHOSPHORUS IN RESPIRING SLICES OF GUINEA PIG CEREBRAL CORTEX: CELLULAR LOCALIZATION OF PHOSPHOPROTEIN SENSITIVE TO ELECTRICAL STIMULATION
Author(s) -
Williams M.,
Pavlik A.,
Rodnight R.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb07602.x
Subject(s) - phosphoprotein , neuropil , stimulation , neuron , biochemistry , phosphate , biology , cerebral cortex , phosphorus , guinea pig , phosphorylation , chemistry , central nervous system , neuroscience , endocrinology , organic chemistry
— In experiments designed to localize the increased turnover of phosphoprotein‐P which occurs in respiring brain slices as a result of electrical stimulation, a cell separation procedure was used to prepare a fraction enriched in neuronal cell bodies from incubated slices labelled with [ 32 P]phosphate. Labelled phosphoprotein was found to be twice as concentrated in the neuron‐enriched fraction as in other fractions. Electrical stimulation for 10 s increased the rate of incorporation of [ 32 P]phosphate into phosphoproteins in the neuron‐enriched fraction by 25 per cent ( P < 0.05), but had no effect on incorporation into a partially purified glial fraction contaminated with neuropil and cell debris.