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SPINAL SUBARACHNOID PERFUSION IN THE RAT: GLYCINE TRANSPORT FROM SPINAL FLUID 1
Author(s) -
Dudzinski D. S.,
Cutler R. W. P.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb07600.x
Subject(s) - taurine , glycine , spinal cord , subarachnoid space , perfusion , cerebrospinal fluid , extracellular fluid , chemistry , amino acid , strychnine , alanine , anesthesia , extracellular , biochemistry , medicine , biology , neuroscience
Abstract— A method was developed for perfusion of the spinal subarachnoid space in the rat. Bidirectional steady‐state fluxes of [ 14 C]glycine between spinal fluid and plasma were measured. [ 14 C]glycine clearance from spinal fluid was 5‐fold greater than its clearance from plasma. Glycine was transported out of spinal fluid by a saturable process, and the rate of transport was unaffected by the other depressant amino acids, GABA, β‐alanine, and taurine. Perfused [ 14 C]glycine and [ 3 H]GABA distributed in an intracellular compartment in spinal cord. The preparation should be useful for study of the release of these inhibitory amino acids from the intact spinal cord.