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THE EFFECTS OF LITHIUM ON HIGH ENERGY PHOSPHATE AND GLUCOSE LEVELS IN THE RAT SUPERIOR CERVICAL GANGLION 1
Author(s) -
Organisciak D. T.,
Klingman J. D.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb07598.x
Subject(s) - phosphocreatine , medicine , endocrinology , lithium (medication) , chemistry , stimulation , superior cervical ganglion , high energy phosphate , ganglion , cervical ganglia , adenosine triphosphate , incubation , biology , biochemistry , anatomy , energy metabolism
— The effects of acute treatment with lithium ion (25 m m ) on the levels of ATP, phosphocreatine and glucose were measured in an in vitro preparation of rat superior cervical ganglion. Similar analyses were performed on ganglia from rats fed a chronic lithium diet (0.5 m m ). Ganglia were excised and de‐sheathed, then stimulated (5 Hz) or rested for 20, 40 or 80 min in normal or lithium‐containing Krebs‐Ringer bathing solutions. Following freeze drying, enzymatic pyridine nucleotide methods were used to measure fluorometrically ATP, phosphocreatine and glucose on portions of a ganglionic perchloric acid extract. After 20 min of incubation, normal ganglionic contents of ATP and phosphocreatine were higher than the initial zero time levels. Incubation for periods longer than 20 min resulted in ATP and phosphocreatine levels which were lower than those at zero time. Stimulation of normal ganglia caused a decrease in the contents of high‐energy phosphates relative to those in the resting state. Except for the 20 min time period the ATP levels were lower than normal in ganglia from rats chronically fed lithium. Levels of ATP and phosphocreatine in ganglia acutely exposed to lithium were significantly lower than in both normal ganglia and ganglia from chronically‐treated rats. In comparison to normal ganglia, significantly lower levels of glucose were found only for stimulated ganglia acutely exposed to lithium.