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ENZYMATIC SULPHATION OF SOME GALACTOSE‐CONTAINING SPHINGOLIPIDS IN DEVELOPING RAT BRAIN 1
Author(s) -
Farrell D. F.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb06937.x
Subject(s) - lactosylceramide , cerebroside , chemistry , sphingolipid , sulfation , biochemistry , enzyme , galactose , fatty acid , moiety , stereochemistry
— Cerebroside sulphotransferase has been found to catalyze the transfer of sulphate from 3′‐phosphoadenosine‐5′‐phosphosulfate (PAPS) to both the α‐hydroxy fatty acid galactosylceramides and the nonhydroxy fatty acid galactosylceramides. The sulphotransferase has a higher affinity for the α‐hydroxy fatty acid galactosylceramides than for the nonhydroxy fatty acid galactosylceramides and will also use lactosylceramide as an acceptor for the transfer of sulphate from PAPS. A second sulphotransferase, PAPS: psychosine sulphotransferase, is also present in the developing rat brain and will catalyse the transfer of sulphate from PAPS to galactosylsphingosine and lactosylsphingosine. The sulphate moiety was determined to be on the galactose and most likely on the 3′ position giving a proposed structure of: 3‐O‐SO 4 ‐galactosylsphingosine. The possible role of this later pathway in the synthesis of sulphogaiactosylceramide remains to be elucidated.