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[ 14 C]GLUTAMATE UPTAKE AND COMPARTMENTATION IN GLIA OF RAT DORSAL SENSORY GANGLION
Author(s) -
Roberts P. J.,
Keen P.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb06935.x
Subject(s) - glutamate receptor , glutamine , biochemistry , biophysics , sensory system , glutamine synthetase , metabolism , biology , sodium , chemistry , neuroscience , amino acid , receptor , organic chemistry
— The characteristics of the uptake of l ‐[U‐ 14 C] glutamate into rat dorsal sensory ganglia were investigated. The uptake was mediated by two distinct kinetic systems, with apparent K m values of the order of 10 −3 M (low affinity) and 10 −5 m (high affinity). The high affinity uptake system was strongly dependent upon temperature and sodium ion concn, and was depressed by a number of metabolic inhibitors. Following uptake, [ 14 C] glutamate was extensively metabolized, primarily to glutamine, although this was not so with cultured ganglia, where in addition to an increased uptake of [ 14 C] glutamate, the specific radioactivity of glutamate was increased and that of glutamine decreased. The labelled substrates [U‐ 14 C]pyruvate and [U‐ 14 C] acetate were used to investigate this phenomenon and the results are discussed in relation to current knowledge of metabolic compartmentation in nervous tissue.