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FLOW IN VIVO OF GLUCOSE CARBON TO BRAIN PROTEIN IN RATS: EFFECT OF STARVATION
Author(s) -
Barkai A.,
Mahadik S.,
Rapport M. M.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb06886.x
Subject(s) - in vivo , starvation , medicine , chemistry , endocrinology , plasma glucose , isoelectric focusing , isoelectric point , blood proteins , biochemistry , biology , insulin , enzyme , microbiology and biotechnology
—The conversion of plasma glucose into brain proteins in vivo was measured in rats after various periods of food deprivation. Rates of flow of glucose carbon into both soluble and insoluble brain proteins were calculated from the curve representing the decrease of plasma [ 14 C]‐glucose specific activity with time, and from the specific activity of brain protein 180 min after intravenous injection of a tracer dose of d ‐[ 14 C]‐glucose. Compared to the post‐absorptive rats, food deprivation for 72 h caused a 30 per cent reduction in the rate of flow of glucose carbon into soluble brain proteins but did not affect the flow into insoluble proteins. Results of experiments in which the soluble brain proteins were separated by isoelectric focusing suggest that prolonged fasting in adult rats causes substantial differences in the conversion of glucose to different proteins.