Premium
DETERGENT‐LIKE ACTION OF TETRAPHENYLBORATE ON PHOSPHOLIPID LABELLING IN GUINEA PIG CORTEX SUBFRACTIONS 1
Author(s) -
Schacht J.,
Agranoff B. W.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb06881.x
Subject(s) - labelling , chemistry , phosphatidic acid , phospholipid , biochemistry , sodium tetraphenylborate , sodium , tetraphenylborate , chromatography , membrane , organic chemistry , ion
—The addition of 2 × 10 −4 m sodium tetraphenylborate to particulate preparations of guinea pig cerebral cortex increases labelling of phosphatidic acid from γ‐[ 32 P]‐ATP two‐ to four‐fold. The effect ‘was observed in all subcellular fractions studied (nuclear, synaptosomal, mitochondrial and microsomal) and is not blocked by the addition of atropine. Changes in phospholipid labelling similar to those induced by tetraphenylborate can be demonstrated with sodium dodecyl sulphate or sodium desoxycholate. It is suggested that tetraphenylborate stimulates lipid labelling by a detergent‐like activation of diglyceride kinase.