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THE EFFECT OF ELEVATED AMINO ACIDS ON AMINOTRANSFERASE LEVELS IN BRAIN AND LIVER OF THE MOUSE
Author(s) -
Benuck M.,
Lajtha A.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb06059.x
Subject(s) - valine , methionine , amino acid , alanine , ornithine , glutamine , lysine , biology , isoleucine , phenylalanine , biochemistry , medicine , arginase , endocrinology , leucine , arginine
— Adult mice were fed standard diets that were enriched with selected amino acids, i.e. 3% methionine, 6% valine, or 8% lysine. These diets caused the following changes in the amino acid pool of the brain measured at 7 and 21 days. The high methionine diet resulted in 50‐fold higher levels of methionine and cysteine and somewhat lower levels of serine and glutamine. The valine and lysine‐enriched diets also caused 2‐ to 4‐fold increases in valine and lysine contents of brain, respectively. In spite of the large changes in amino acid levels, however, there were essentially no changes in aspartate: α‐ketoglutarate, alanine: α‐ketoglutarate, ornithine: α‐ketoglutarate, methionine: α‐ketoglutarate, and the branched chain aminotransferase activities of brain 3, 10, and 21 days after the onset of the dietary regimen. In contrast, these diets produced significant changes in some of these enzyme activities in liver. Changes in liver included a 2‐fold increase in ornithine and alanine aminotransferase activities with the methionine‐enriched diet. Liver ornithine aminotransferase activity also increased slightly in animals fed the valine‐enriched or lysine‐enriched diet.