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RNA METABOLISM IN RABBIT BRAIN: STUDY WITH NEURON‐GLIA AND SUBCELLULAR FRACTIONS 1
Author(s) -
Yanagihara T.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb04410.x
Subject(s) - ficoll , rna , centrifugation , biochemistry , differential centrifugation , neuron , microsome , incubation , uridine , leucine , metabolism , nucleotide , biology , cell fractionation , ribosomal rna , in vitro , nucleic acid , amino acid , enzyme , gene , peripheral blood mononuclear cell , neuroscience
—Uridine incorporation into RNA of rabbit brain was studied by using an in vitro system for incubation of brain slices for up to 180 min. Neuron‐enriched and glia‐enriched fractions were prepared by ficoll density gradient centrifugation, and various subcellular fractions were prepared by sucrose density gradient centrifugation. Although the difference was not as great as in the case of l ‐leucine incorporation into protein, the neuron‐enriched fraction consistently showed a higher specific radioactivity than the glia‐enriched fraction. The specific radioactivity of the nuclei increased promptly and remained high at 180 min; the increase in the microsomes was gradual. Comparison of these data suggests that both neuron‐enriched and glia‐enriched fractions retain high radioactivities in their nuclei at 180 min when a considerable portion of the ribosomal RNA in these fractions is not labeled. The sharp diffusion gradient of nucleotides is discussed in relation to the acid‐soluble radioactivity.