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PHOSPHOLIPID METABOLISM IN EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS: ACTIVITY OF BRAIN PHOSPHOLIPASE A 1 , TOWARDS SPECIFICALLY LABELLED GLYCEROPHOSPHOLIPIDS
Author(s) -
Woelk H.,
Kanig K.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb04398.x
Subject(s) - glycerophospholipids , phosphatidylcholine , phospholipid , phospholipase , biochemistry , enzyme , phospholipase a2 , encephalomyelitis , chemistry , ethanolamine , phospholipase a , enzyme assay , plasmalogen , biology , endocrinology , central nervous system , membrane
—The activity of phospholipase A 1 (phosphatide acylhydrolase, EC 3.1.1.4) was measured against phosphatidylcholine, ‐ethanolamine and ‐serine, specifically labelled with different fatty acids at either the 1 or 2 position, during experimental allergic encephalomyelitis in rats. In the acute stage of the disease there was a significant increase in enzyme activity in comparison to that from the control animals. The enhanced phospholipase A 1 activity was of the‐same order of magnitude for all 1,2‐diacyl‐glycerophospholipids investigated. The phospholipase A 1 ‐catalysed release of labelled fatty acids from molecular species of phosphatidylcholine decreased slightly with increasing degree of unsaturation of the fatty acids at the 1‐position. The K m ‐values of the highly purified enzyme were not altered by the demyelinating disorder. The phospholipase A 1 activity returned to the control level in the recovery stage.