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REACTIONS OF FREE AND tRNA BOUND GLUTAMATE AND GLUTAMINE
Author(s) -
Murthy M. R. V.,
Roux Huguette
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb04387.x
Subject(s) - glutamine , transfer rna , amino acid , biochemistry , glutamate receptor , chemistry , glutamic acid , rna , receptor , gene
Abstract —[ 14 C]‐Glutamate and [ 14 C]‐glutamine were incorporated into calf brain tRNA in the presence of homologous aminoacyl‐tRNA synthetases. When the tRNAs were then deaminoacylated and chromatographed, a number of radioactive products were found in addition to the original amino acids. One of the products of glutamate transformation was identified to be glutamine. Formation of the radioactive products of glutamate in the presence and absence of tRNA indicated that glutamine was produced from glutamate at the level of the free amino acid followed by the incorporation of both substances into tRNA. Examination of the products of deaminoacylation of glutaminyl‐tRNA showed that glutamine underwent structural alterations at the level of the aminoacyl‐tRNAs to give rise to a cyclic derivative of glutarimide. This reaction was specific for glutamine, and constituted approximately 15 per cent of the total radioactivity in the deaminoacylation products of glutaminyl‐tRNA.