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ON THE MECHANISM OF STIMULATION OF AMP‐AMINOHYDROLASE ACTIVITY BY HEXOKINASE IN BRAIN TISSUE
Author(s) -
Haroutunian A. V.,
Buniatian H. Ch.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb04357.x
Subject(s) - hexokinase , biochemistry , enzyme , yeast , sephadex , chemistry , stimulation , urea , enzyme assay , biology , glycolysis , endocrinology
—A hexokinase has been isolated from brain tissue on Sephadex G‐100 and DEAE cellulose which is similar to yeast enzyme in stimulating the AMP‐aminohydrolase activity of rat brain soluble fractions. This effect of hexokinase is influenced neither by N ‐acetyl‐glucosamine nor noradrenaline. An isoenzyme of hexokinase isolated from brain tissue on DEAE cellulose, having properties similar to that of the muscle enzyme, has no effect on AMP‐aminohydrolase activity. The activating effect of yeast hexokinase is not due to its oligomeric structure. Enzyme subunits obtained by the treatment of native yeast enzyme by urea also activate AMP‐aminohydrolase of rat brain soluble fractions.